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icb elisa prism 6 package  (GraphPad Software Inc)

 
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    Structured Review

    GraphPad Software Inc icb elisa prism 6 package
    A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the <t>ICB-ELISA.</t> While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.
    Icb Elisa Prism 6 Package, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icb elisa prism 6 package/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    icb elisa prism 6 package - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Detection of Serotype-Specific Antibodies to the Four Dengue Viruses Using an Immune Complex Binding (ICB) ELISA"

    Article Title: Detection of Serotype-Specific Antibodies to the Four Dengue Viruses Using an Immune Complex Binding (ICB) ELISA

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0002580

    A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the ICB-ELISA. While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.
    Figure Legend Snippet: A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the ICB-ELISA. While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.

    Techniques Used: Incubation, Labeling, Recombinant, Enzyme-linked Immunosorbent Assay



    Similar Products

    icb elisa prism 6 package  (GraphPad Software Inc)
    90
    GraphPad Software Inc icb elisa prism 6 package
    A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the <t>ICB-ELISA.</t> While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.
    Icb Elisa Prism 6 Package, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icb elisa prism 6 package/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    icb elisa prism 6 package - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the ICB-ELISA. While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Detection of Serotype-Specific Antibodies to the Four Dengue Viruses Using an Immune Complex Binding (ICB) ELISA

    doi: 10.1371/journal.pntd.0002580

    Figure Lengend Snippet: A : During incubation of serum samples containing antibody (AB) and POD-labeled recombinant antigen (POD, AG) immune complexes form, which in turn are attached to the solid-phase coated with CD32. Non-specific antibodies (in grey) do neither bind to the antigen nor to CD32. Competition with unlabeled antigen is not shown. B : Schematic drawing of the POD-labeled EDIII domain of DENV-2 (DeP2) and of the unlabeled EDIII domain of DENV-3 (De3) serving as competitor in the ICB-ELISA. While type-specific epitopes on the DeP2 and De3 are different from each other, cross-reacting epitopes are almost identical. In the presence of an excess of De3, cross-reacting antibodies will no longer bind to sub-domain epitopes on the DeP2 antigen. Amino acids (aa) involved in epitope formation are also indicated. Of note, a subcomplex (aa 305,307) and a type-specific (aa 303,304) epitope are in close proximity.

    Article Snippet: Intra- and interassay coefficients of variation (CVs) were calculated for each ICB ELISA ((Prism 6 Package, GraphPad Software, Inc., San Diego, CA) using our four positive control sera in rows of sixfold, tested on three consecutive days.

    Techniques: Incubation, Labeling, Recombinant, Enzyme-linked Immunosorbent Assay